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5 ethynyl uridine  (Jena Bioscience)


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    Structured Review

    Jena Bioscience 5 ethynyl uridine
    5 Ethynyl Uridine, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5+ethynyl+uridine/5-Ethynyl-uridine/bio_rxiv__64898__2026__04__24__718482-294-5-7
    Average 96 stars, based on 93 article reviews
    5 ethynyl uridine - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Irradiation:

    Article Title: Hierarchical mechanisms control the clearance of DNA lesion–stalled RNA polymerase II
    Article Snippet: .. Cells were irradiated with UV-C light (9 J/m 2 ) or not and incubated for the indicated periods, then pulse-labeled with 400 μM 5-ethynyl-uridine (EU; Jena Bioscience) for 1 h followed by a 15 min medium-chase with DMEM without supplements. .. Cells were fixed with 3.7% formaldehyde in phosphate-buffered saline solution (PBS) for 15 min, permeabilized with 0.5% Triton X-100 in PBS for 10 min at room temperature, and blocked in 1.5% bovine serum albumin (BSA, Thermo Fisher) in PBS.

    Article Title: CFAP20 salvages arrested RNAPII from the path of co-directional replisomes.
    Article Snippet: .. Cells were irradiated with UV-C light (12 J m−2), allowed to recover for the indicated periods and pulse-labelled with 400 μM 5-ethynyl-uridine (EU; Jena Bioscience) for one hour, followed by a 15 min medium-chase with DMEM without supplements. .. Cells were fixed with 3.7% formaldehyde in PBS for 15 min, permeabilized with 0.5% Triton X-100 in PBS for 10 min at room temperature and blocked in 1.5% BSA (Thermo Fisher Scientific) in PBS.

    Article Title: CFAP20 salvages arrested RNAPII from the path of co-directional replisomes
    Article Snippet: .. Cells were irradiated with UV-C light (12 J m −2 ), allowed to recover for the indicated periods and pulse-labelled with 400 μM 5-ethynyl-uridine (EU; Jena Bioscience) for one hour, followed by a 15 min medium-chase with DMEM without supplements. .. Cells were fixed with 3.7% formaldehyde in PBS for 15 min, permeabilized with 0.5% Triton X-100 in PBS for 10 min at room temperature and blocked in 1.5% BSA (Thermo Fisher Scientific) in PBS.

    Incubation:

    Article Title: Hierarchical mechanisms control the clearance of DNA lesion–stalled RNA polymerase II
    Article Snippet: .. Cells were irradiated with UV-C light (9 J/m 2 ) or not and incubated for the indicated periods, then pulse-labeled with 400 μM 5-ethynyl-uridine (EU; Jena Bioscience) for 1 h followed by a 15 min medium-chase with DMEM without supplements. .. Cells were fixed with 3.7% formaldehyde in phosphate-buffered saline solution (PBS) for 15 min, permeabilized with 0.5% Triton X-100 in PBS for 10 min at room temperature, and blocked in 1.5% bovine serum albumin (BSA, Thermo Fisher) in PBS.

    Article Title: mRNA poly(A)-tail length is a battleground for coronavirus–host competition
    Article Snippet: For RNA analysis, RNA was extracted directly from beads using TRIzol, whereas for protein analysis, beads were boiled in 1× LDS sample buffer (Invitrogen). .. To label nascent transcripts, infected cells were incubated with 200 μM 5-ethynyl uridine (5-EU; Jena Biosciences) for 10 min. ..

    Purification:

    Article Title: RNA m 6 A regulates the transcription and heterochromatin state of retrotransposons in Arabidopsis
    Article Snippet: .. Six-day-old WT and cpsf30-l seedlings were placed in six-well plates containing 0.5 mM 5-ethynyl uridine (Jena Bioscience) and were collected at 30 min, 60 min and 120 min. Total RNA was purified using Trizol followed by rRNA depletion; nascent rRNA-depleted RNA was captured using a Click-iT Nascent RNA Capture Kit (Invitrogen). .. Before constructing libraries with a NEBNext Ultra II Directional RNA Library Prep Kit (NEB), an equal amount of ERCC RNA spike-in control (Ambion) was added to each sample.

    Infection:

    Article Title: mRNA poly(A)-tail length is a battleground for coronavirus–host competition
    Article Snippet: For RNA analysis, RNA was extracted directly from beads using TRIzol, whereas for protein analysis, beads were boiled in 1× LDS sample buffer (Invitrogen). .. To label nascent transcripts, infected cells were incubated with 200 μM 5-ethynyl uridine (5-EU; Jena Biosciences) for 10 min. ..

    Labeling:

    Article Title: Nucleolar reorganization on stress depends on physicochemical changes due to nascent rRNA synthesis
    Article Snippet: .. For labeling the RNA, the cells were pulse labeled with 5-Ethynyl uridine (5-EU, CLK-N002-10, Jena Biosciences) for 1mM for 30 minutes and then click chemistry was performed to visualize them. ..

    Article Title: ProPER: Programmable, multiplexed detection of molecular proximities and RNA life-cycle stages in situ
    Article Snippet: .. For the metabolic labeling experiments 5-ethynyl uridine (Jena Bioscience, CLK-N002-10) was added 30 min before fixation to a final concentration of 1 mM. ..

    Concentration Assay:

    Article Title: ProPER: Programmable, multiplexed detection of molecular proximities and RNA life-cycle stages in situ
    Article Snippet: .. For the metabolic labeling experiments 5-ethynyl uridine (Jena Bioscience, CLK-N002-10) was added 30 min before fixation to a final concentration of 1 mM. ..



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